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Antibody staining overlaps with antibody HPA071089.
Validated
Antibody staining overlaps with antibody HPA003154.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Antibody dilution
1:10
1:96
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003154
Antibody HPA071089
Antibody CAB009324
Antibody CAB013645
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human gall bladder shows distinct cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 72 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:25
1:20
1:3000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003154
Antibody HPA071089
Antibody CAB009324
Antibody CAB013645
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401861)
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
82.6, 82.1
82.6, 82.1
82.6, 82.1, 20, 18, 15.2, 5
82.6, 82.1, 20, 18, 15.2, 5
Antibody dilution
1:250
1:320
1:500
1:500
PROTEIN ARRAY
Antibody HPA003154
Antibody HPA071089
Antibody CAB009324
Antibody CAB013645
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
