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Antibody staining overlaps with antibody HPA032045.
Validated
Antibody staining overlaps with antibody HPA031707.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Antibody dilution
1:21
1:91
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031707
Antibody HPA031708
Antibody HPA032045
Antibody CAB017840
Standard validation
Approved
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 42 cell types
RNA: detected in 37 tissues Protein: detected in 52 cell types
RNA: detected in 37 tissues Protein: detected in 35 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:35
1:200
1:250
1:800
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031707
Antibody HPA031708
Antibody HPA032045
Antibody CAB017840
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.2, 22.3, 18.3
26.2, 19.7
26.2, 22.3, 19.7, 18.3
26.2, 22.3, 19.7, 18.3
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA031707
Antibody HPA031708
Antibody HPA032045
Antibody CAB017840
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
