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ATP2A3

ANTIBODY INFORMATION
 
 

Antibody HPA007180

  

Antibody CAB010882

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA007180   A7952  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 4   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA007180

  

Antibody CAB010882

  
Immunohistochemistry


Validated
   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007180

  

Antibody CAB010882

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nucleolar and cytoplasmic positivity in purkinje cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:80   1:350  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007180

  

Antibody CAB010882

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 114.9, 114, 112.5, 109.3, 109.1   114.9, 114, 112.5, 109.3, 109.1, 17.2, 15.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007180

  

Antibody CAB010882

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA007180

  

Antibody CAB010882

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 101    

Antigen sequence

 
SRDRKSMSVYCTPTRPHPTGQGSKMFVKGAPESVIERCSSVRVGSRTAPL
TPTSREQILAKIRDWGSGSDTLRCLALATRDAPPRKEDMELDDCSKFVQY
E
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATP2A3-001 - ENSP00000380234 [100%] 
ATP2A3-002 - ENSP00000380236 [100%] 
ATP2A3-004 - ENSP00000380229 [100%] 
ATP2A3-005 - ENSP00000353072 [100%] 
ATP2A3-015 - ENSP00000301387 [100%] 
ATP2A3-201 - ENSP00000312577 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP2A3-001
ATP2A3-002
ATP2A3-004
ATP2A3-005
ATP2A3-015
ATP2A3-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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