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NUAK1

ANTIBODY INFORMATION
 
 

Antibody HPA027455

  

Antibody HPA057143

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027455   HPA057143  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA027455

  

Antibody HPA057143

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Supported   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027455

  

Antibody HPA057143

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli fibrillar center & microtubules.  

Antibody dilution

   1:40  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027455

  

Antibody HPA057143

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 56 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:10    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA027455

  

Antibody HPA057143

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 74.3   74.3  

Antibody dilution

 1:250   1:130  

PROTEIN ARRAY
 
 

Antibody HPA027455

  

Antibody HPA057143

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1600  

ANTIGEN INFORMATION
 
 

Antibody HPA027455

  

Antibody HPA057143

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   70  

Antigen sequence

 
LSRSYSRPSSVISDDSVLSSDSFDLLDLQENRPARQRIRSCVSAENFLQI
QDFEGLQNRPRPQYLKRYRNRLADSSFSLLTDMDDVTQVYKQ
  
LLDSNDVMGSSIPSPSPPDPARVTSHSLSCRRKGILKHSSKYSAGTMDPA
LVSPEMPTLESLSEPGVPAE
 

Matching transcripts

 NUAK1-001 - ENSP00000261402 [100%]
  NUAK1-001 - ENSP00000261402 [100%]
 

Other gene match

    
ANTIGEN VIEW
NUAK1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.