Fields »
ABOUT
HELP
BLOG
SLC12A1

ANTIBODY INFORMATION
 
 

Antibody HPA014967

  

Antibody HPA018107

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014967   HPA018107  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA014967

  

Antibody HPA018107

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014967

  

Antibody HPA018107

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubular cells.   Immunohistochemical staining of human kidney shows strong membranous positivity in cells of distal tubules.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 2 cell types		   RNA: detected in 3 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014967

  

Antibody HPA018107

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 121.4, 46.5, 24.3   121.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA014967

  

Antibody HPA018107

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA014967

  

Antibody HPA018107

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 121   104  

Antigen sequence

 
MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSF
GDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQT
FGHNTMDAVPKIEYYRNTGSI
  
SQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAG
KLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWW
LFDD
 

Matching transcripts

 SLC12A1-001 - ENSP00000379822 [100%]
SLC12A1-002 - ENSP00000331550 [100%]
SLC12A1-006 - ENSP00000453409 [100%]
SLC12A1-011 - ENSP00000454178 [100%]
SLC12A1-201 - ENSP00000370381 [100%]
  SLC12A1-001 - ENSP00000379822 [100%]
SLC12A1-006 - ENSP00000453409 [100%]
SLC12A1-201 - ENSP00000370381 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC12A1-001
SLC12A1-002
SLC12A1-006
SLC12A1-011
SLC12A1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.