SRI - Antibodies - The Human Protein Atlas

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SRI

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA019004

Antibody HPA073666

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA019004

HPA073666

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA019004

Antibody HPA073666

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA019004

Antibody HPA073666

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Antibody dilution

1:6

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA019004

Antibody HPA073666

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA073666 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA019004 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 18 cell types

RNA: detected in 37 tissues
Protein: detected in 30 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:5000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA019004

Antibody HPA073666

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

21.7, 20.3, 20, 17.1

21.7, 20.3, 20, 17.6

Antibody dilution

1:250

1:590

PROTEIN ARRAY

Antibody HPA019004

Antibody HPA073666

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:7050

ANTIGEN INFORMATION

Antibody HPA019004

Antibody HPA073666

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

GGGYYPGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLT
QSGIAGGYK

LNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQAVNSIAKRYSTN
GKITFDDYI

Matching transcripts

SRI-001 - ENSP00000265729 [100%]
SRI-005 - ENSP00000397609 [100%]
SRI-003 - ENSP00000391148 [86%]
SRI-012 - ENSP00000378137 [86%]

SRI-001 - ENSP00000265729 [100%]
SRI-003 - ENSP00000391148 [100%]
SRI-004 - ENSP00000418512 [100%]
SRI-012 - ENSP00000378137 [100%]

Other gene match

ANTIGEN VIEW

SRI-001	SRI-003	SRI-004	SRI-005	SRI-012

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.