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TOP2B

ANTIBODY INFORMATION
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA024120   HPA050441   sc-25330  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   12   2  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  
Immunocytochemistry


Validated
   Supported   Supported  
Immunohistochemistry

Supported     Supported  
Western blot


Validated
   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

 

Relative fluorescence intensity (RFI)

      

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:105      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus.  

Antibody dilution

 1:100   1:64   1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human pancreas shows moderate nuclear positivity.     Immunohistochemical staining of human pancreas shows strong nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		     RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:300     1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 183.3, 182.7      

Loading control

 Total protein image      

Antibody dilution

 1:233      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 183.3, 182.7   183.3, 182.7   183.3, 182.7, 130.5  

Antibody dilution

 1:250   1:320   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:4000    

ANTIGEN INFORMATION
 
 

Antibody HPA024120

  

Antibody HPA050441

  

Antibody CAB004601

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 143   138    

Antigen sequence

 
LWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEET
MPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPV
EGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSA
  
DKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKT
TTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVD
IFPSDFPTEPPSLPRTGRARKEVKYFAESDEEEDDVDF
  

											 
																				

																		

										
											
Matching transcripts

												
																					TOP2B-001 - ENSP00000396704 [100%] 
TOP2B-201 - ENSP00000264331 [100%] 

											 
																						TOP2B-001 - ENSP00000396704 [100%] 
TOP2B-201 - ENSP00000264331 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TOP2B-001
TOP2B-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA024120

												
										 
																				
											
											
											
Antibody HPA050441

												
										 
																				
											
											
											
Antibody CAB004601

												
										 
																		

																	

										
											
1.

												
																						Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis

	Aviner R et al

	PLoS Genet 2015;11(10):e1005554

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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