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NFKB2

ANTIBODY INFORMATION
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA008422   HPA023900   05-361  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   5   5  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry


Validated
  

Validated

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.     Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:38     1:100  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 
 

 

    

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA023900 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA008422 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.   Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in most cells.   Immunohistochemical staining of human pancreas shows strong positivity in enocrine and exocrine cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:40   1:15   1:400  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 96.7   96.7, 45.6   96.7, 45.6, 7.4, 2.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA008422

  

Antibody HPA023900

  

Antibody CAB022098

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 131   135    

Antigen sequence

 
LLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELA
ERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVR
LLRGPETRDKLPSTEVKEDSAYGSQSVEQEA
  
ICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSELGICAVSVGPKDMTA
QFNNLGVLHVTKKNMMGTMIQKLQRQRLRSRPQGLTEAEQRELEQEAKEL
KKVMDLSIVRLRFSAFLRASDGSFSLPLKPVISQP
  

											 
																				

																		

										
											
Matching transcripts

												
																					NFKB2-001 - ENSP00000189444 [100%] 
NFKB2-201 - ENSP00000410256 [100%] 
NFKB2-003 - ENSP00000358983 [99%] 

											 
																						NFKB2-001 - ENSP00000189444 [100%] 
NFKB2-003 - ENSP00000358983 [100%] 
NFKB2-201 - ENSP00000410256 [100%] 
NFKB2-202 - ENSP00000480211 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NFKB2-001
NFKB2-003
NFKB2-201
NFKB2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008422

												
										 
																				
											
											
											
Antibody HPA023900

												
										 
																				
											
											
											
Antibody CAB022098

												
										 
																		

																	

										
											
1.

												
																						A roadmap of constitutive NF-κB activity in Hodgkin lymphoma: Dominant roles of p50 and p52 revealed by genome-wide analyses

	de Oliveira KA et al

	Genome Med 2016;8(1):28

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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