FGFR1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

FGFR1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA056402

HPA076274

sc-57132

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line BJ shows localization to nucleoli.

Antibody dilution

1:7

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.

Immunohistochemical staining of human kidney shows cytoplasmic, membranous and nuclear positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 37 cell types

RNA: detected in 37 tissues
Protein: detected in 69 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:25

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

95.3, 91.9, 91.7, 91.6, 90.6, 40.7, 33.3, 11.9

95.3, 91.9, 91.7, 91.6, 90.6, 82.2, 81.9

95.3, 91.9, 91.7, 91.6, 90.6, 82.2, 82, 81.9, 40.7, 33.3, 25.8, 16.4, 16, 15.7, 11.9, 9.5, 6.2, 0.1

Antibody dilution

1:230

1:80

1:500

PROTEIN ARRAY

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA056402

Antibody HPA076274

Antibody CAB033614

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

LPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAES
NRTR

AWLTVLEALEERPAVMTSPLYLEI

Matching transcripts

FGFR1-001 - ENSP00000380280 [100%]
FGFR1-002 - ENSP00000432972 [100%]
FGFR1-012 - ENSP00000380302 [100%]
FGFR1-015 - ENSP00000400708 [100%]
FGFR1-020 - ENSP00000380297 [100%]
FGFR1-036 - ENSP00000434712 [100%]
FGFR1-201 - ENSP00000340636 [100%]
FGFR1-202 - ENSP00000393312 [100%]
FGFR1-203 - ENSP00000400162 [100%]
FGFR1-003 - ENSP00000337247 [96%]

FGFR1-001 - ENSP00000380280 [100%]
FGFR1-002 - ENSP00000432972 [100%]
FGFR1-003 - ENSP00000337247 [100%]
FGFR1-004 - ENSP00000348537 [100%]
FGFR1-008 - ENSP00000327229 [100%]
FGFR1-012 - ENSP00000380302 [100%]
FGFR1-020 - ENSP00000380297 [100%]
FGFR1-202 - ENSP00000393312 [100%]
FGFR1-203 - ENSP00000400162 [100%]

Other gene match

ANTIGEN VIEW

FGFR1-001	FGFR1-002	FGFR1-003	FGFR1-004	FGFR1-008	FGFR1-012	FGFR1-015	FGFR1-020	FGFR1-036	FGFR1-201	FGFR1-202	FGFR1-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.