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ANTIBODY INFORMATION
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA008273   HPA012828   05-346  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   4   1  

References

 1   2    

VALIDATION SUMMARY
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA012828.		  
Validated

Antibody staining overlaps with antibody HPA008273.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.    

Antibody dilution

 1:30   1:16    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

  
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA012828 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA008273 across 45 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells and glial cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 30 cell types		   RNA: detected in 35 tissues
Protein: detected in 19 cell types		   RNA: detected in 35 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:80   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  
 

Standard validation

   Supported    

     

Figure description

   Immunoreactivity observed in neuronal cell bodies and dendrites in various areas of the brain.    

WESTERN BLOT
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 199.5, 199.1, 59, 49.6   199.5, 199.1, 61.8, 59, 49.6   199.5, 199.1, 61.8, 59, 49.6, 21.8, 11.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA008273

  

Antibody HPA012828

  

Antibody CAB001984

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 135   141    

Antigen sequence

 
SLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSG
TSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVP
SEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVK
  
EAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGA
FGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLK
GEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVT
  

											 
																				

																		

										
											
Matching transcripts

												
																					MAP2-001 - ENSP00000353508 [100%] 
MAP2-002 - ENSP00000376032 [100%] 
MAP2-003 - ENSP00000199940 [100%] 
MAP2-014 - ENSP00000392164 [100%] 
MAP2-015 - ENSP00000355290 [100%] 

											 
																						MAP2-001 - ENSP00000353508 [100%] 
MAP2-002 - ENSP00000376032 [100%] 
MAP2-003 - ENSP00000199940 [100%] 
MAP2-014 - ENSP00000392164 [100%] 
MAP2-015 - ENSP00000355290 [100%] 
MAP2-016 - ENSP00000409969 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MAP2-001
MAP2-002
MAP2-003
MAP2-014
MAP2-015
MAP2-016
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008273

												
										 
																				
											
											
											
Antibody HPA012828

												
										 
																				
											
											
											
Antibody CAB001984

												
										 
																		

																	

										
											
1.

												
																						Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche

	Lee DA et al

	Nat Neurosci 2012;15(5):700-2

			
											 
																							The Human Protein Atlas--a tool for pathology

	Pontén F et al

	J Pathol 2008;216(4):387-93

			
											 
																						
											 
																				

																		

										
											
2.

												
																					
											 
																							Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection

	Andersson S et al

	J Histochem Cytochem 2013;61(11):773-84

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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