We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human colon shows distinct positivity in peripheral nerve/ganglion.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells, cells of molecular and granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells and granular layer while additional nuclear positivity in molecular layer.
Expression
RNA: detected in 22 tissues Protein: detected in 29 cell types
RNA: detected in 22 tissues Protein: detected in 29 cell types
RNA: detected in 22 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:1800
1:4000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019828
Antibody HPA019829
Antibody CAB008559
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
76.3, 71.9, 61.5
76.3, 71.9, 61.5
76.3, 71.9, 61.5
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA019828
Antibody HPA019829
Antibody CAB008559
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
