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ARAF

ANTIBODY INFORMATION
 
 

Antibody HPA046188

  

Antibody HPA066326

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046188   HPA066326  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA046188

  

Antibody HPA066326

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046188

  

Antibody HPA066326

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & nucleoli fibrillar center.    

Antibody dilution

 1:10    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046188

  

Antibody HPA066326

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:150  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046188

  

Antibody HPA066326

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.9, 67.6   67.9, 67.6  

Antibody dilution

 1:250   1:220  

PROTEIN ARRAY
 
 

Antibody HPA046188

  

Antibody HPA066326

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:2700  

ANTIGEN INFORMATION
 
 

Antibody HPA046188

  

Antibody HPA066326

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 72   69  

Antigen sequence

 
DSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAE
QRERKSLADDKKKVKNLGYRDS
  
MSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEH
FPFPAPANAPLQRIRSTST
 

Matching transcripts

 ARAF-001 - ENSP00000366244 [100%]
ARAF-201 - ENSP00000290277 [100%]
  ARAF-001 - ENSP00000366244 [100%]
ARAF-201 - ENSP00000290277 [96%]
 

Other gene match

    
ANTIGEN VIEW
ARAF-001
ARAF-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.