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TNS1

ANTIBODY INFORMATION
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA036089   HPA036090   sc-28542  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 8   8   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Approved   Approved   Approved  
Western blot


Validated
   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA036090.		  
Validated

Antibody staining overlaps with antibody HPA036089.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.   Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.    

Antibody dilution

 1:80   1:23    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in tubules and cells in glomeruli.   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules and cells in glomeruli.   Immunohistochemical staining of human kidney shows cytoplasmic and basement membranous positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 21 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:15   1:80  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 185.7, 184.2, 183.2, 143.5, 85.7      

Loading control

 Total protein image      

Antibody dilution

 1:178      
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 185.7, 184.2, 183.2, 143.5, 85.7   185.7, 184.2, 183.2, 143.5, 89   185.7, 184.2, 183.2, 143.5, 89, 85.7, 53.8, 47.2, 16.5, 13.3, 11.8, 6.9, 2.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA036089

  

Antibody HPA036090

  

Antibody CAB018774

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 85   88    

Antigen sequence

 
LSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQE
GLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQS
  
EEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATS
PRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYK
  

											 
																				

																		

										
											
Matching transcripts

												
																					TNS1-001 - ENSP00000171887 [100%] 
TNS1-002 - ENSP00000405460 [100%] 
TNS1-004 - ENSP00000408724 [100%] 
TNS1-005 - ENSP00000406016 [100%] 
TNS1-201 - ENSP00000484271 [100%] 
TNS1-202 - ENSP00000480559 [100%] 

											 
																						TNS1-001 - ENSP00000171887 [100%] 
TNS1-004 - ENSP00000408724 [100%] 
TNS1-005 - ENSP00000406016 [100%] 
TNS1-201 - ENSP00000484271 [100%] 
TNS1-202 - ENSP00000480559 [100%] 
TNS1-009 - ENSP00000394171 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TNS1-001
TNS1-002
TNS1-004
TNS1-005
TNS1-009
TNS1-201
TNS1-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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