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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Antibody dilution
1:22
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA002353
Standard validation
Approved
Figure description
Immunohistochemical staining of human breast shows nuclear membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002353
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414965)
Target mass (kDa)
73.6
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA002353
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
