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AP4E1

ANTIBODY INFORMATION
 
 

Antibody HPA041749

  

Antibody HPA041891

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041749   HPA041891  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041749

  

Antibody HPA041891

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041749

  

Antibody HPA041891

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 54 cell types		   RNA: detected in 35 tissues
Protein: detected in 25 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:150  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041749

  

Antibody HPA041891

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 127.3, 119.5   127.3, 119.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041749

  

Antibody HPA041891

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041749

  

Antibody HPA041891

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 116   103  

Antigen sequence

 
LMVWSVTNKSGLELKSADLEIFPAENFKVTEQPGCCLPVMEAESTKSFQY
SVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVNLSLLDFIRPLKISSDD
FGKLWLSFANDVKQNV
  
AELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYL
GLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQN
ITV
 

Matching transcripts

 AP4E1-001 - ENSP00000261842 [100%]
AP4E1-002 - ENSP00000452976 [100%]
  AP4E1-001 - ENSP00000261842 [100%]
AP4E1-002 - ENSP00000452976 [100%]
 

Other gene match

    
ANTIGEN VIEW
AP4E1-001
AP4E1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.