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MEF2C

ANTIBODY INFORMATION
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005533   AMAb90728   AMAb90727  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 3   13   13  

References

 4      

VALIDATION SUMMARY
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  
Immunocytochemistry

Supported      
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.      

Antibody dilution

 1:8      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

  
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 16 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:375   1:1000   1:2000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419349)		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52.3, 51.2, 51.1, 50.3, 50.2, 47.9, 47, 46.9, 44.9, 43.1, 42.5   52.3, 51.2, 51.1, 50.3, 50.2, 47.9, 47, 46.9, 44.9, 43.1, 42.5, 26.6, 25, 22.9, 21.8, 18.9, 15.9, 14.9, 14.6, 14.1, 10.5, 10.3, 3.5   52.3, 51.2, 51.1, 50.3, 50.2, 47.9, 47, 46.9, 44.9, 43.1, 42.5, 26.6, 25, 22.9, 21.8, 18.9, 15.9, 14.9, 14.6, 14.1, 10.5, 10.3, 3.5  

Antibody dilution

 1:250   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA005533

  

Antibody CAB068196

  

Antibody CAB068197

  

Antigen

 Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 139      

Antigen sequence

 
PPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVT
HRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQ
AKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINN
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					MEF2C-004 - ENSP00000389610 [100%] 
MEF2C-005 - ENSP00000340874 [100%] 
MEF2C-007 - ENSP00000486554 [100%] 
MEF2C-009 - ENSP00000486490 [100%] 
MEF2C-022 - ENSP00000422390 [100%] 
MEF2C-024 - ENSP00000423597 [100%] 
MEF2C-026 - ENSP00000487430 [100%] 
MEF2C-032 - ENSP00000487311 [100%] 
MEF2C-001 - ENSP00000421925 [95%] 
MEF2C-003 - ENSP00000396219 [95%] 
MEF2C-023 - ENSP00000424606 [95%] 
MEF2C-025 - ENSP00000425636 [95%] 
MEF2C-028 - ENSP00000426665 [95%] 
MEF2C-029 - ENSP00000487538 [95%] 
MEF2C-031 - ENSP00000487184 [95%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

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MEF2C-001
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MEF2C-004
MEF2C-005
MEF2C-007
MEF2C-009
MEF2C-022
MEF2C-023
MEF2C-024
MEF2C-025
MEF2C-026
MEF2C-028
MEF2C-029
MEF2C-031
MEF2C-032
								

							

						

							
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RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA005533

												
										 
																				
											
											
											
Antibody CAB068196

												
										 
																				
											
											
											
Antibody CAB068197

												
										 
																		

																	

										
											
1.

												
																						MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological disease

	Yelamanchili SV et al

	Cell Death Dis 2010;1:e77

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Differential expression of cartilage and bone-related proteins in pediatric and adult diseased aortic valves

	Wirrig EE et al

	J Mol Cell Cardiol 2011;50(3):561-9

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Mef2c regulates transcription of the extracellular matrix protein cartilage link protein 1 in the developing murine heart

	Lockhart MM et al

	PLoS One 2013;8(2):e57073

			
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Evolutionary conservation of Nkx2.5 autoregulation in the second heart field

	Clark CD et al

	Dev Biol 2013;374(1):198-209

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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