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ANTIBODY INFORMATION
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   DakoCytomation
  

Product name

 HPA004751   HPA008428   HPA017176   NCL-PGR   M3569  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Supernatant  

Other gene match

          

Released in version

 3   3   4   1   12  

References

 1   1        

VALIDATION SUMMARY
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  
Immunohistochemistry

Supported   Supported   Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human corpus, uterine shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human endometrium shows strong nuclear positivity.   Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human uterus, post-menopause shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 20 tissues
Protein: detected in 39 cell types		   RNA: detected in 20 tissues
Protein: detected in 16 cell types		   RNA: detected in 20 tissues
Protein: detected in 51 cell types		   RNA: detected in 20 tissues
Protein: detected in 35 cell types		   RNA: detected in 20 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:250   1:15   1:15   1:100   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

         

Figure description

          

Target mass (kDa)

 99, 79.3, 38.8   99, 87.7, 79.3, 71.2   99, 87.7, 79.3, 71.2   99, 87.7, 79.3, 71.2, 38.8, 19   99, 87.7, 79.3, 71.2, 38.8, 19  

Antibody dilution

 1:250   1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000   1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known   Native protein  

Length (aa)

 90   97   150      

Antigen sequence

 
RVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIE
PDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKS
  
LLEDESYDGGAGAASAFAPPRSSPCASSTPVAVGDFPDCAYPPDAEPKDD
AYPLYSDFQPPALKIKEEEEGAEASARSPRSYLVAGANPAAFPDFPL
  
TELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAI
PISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSS
SPPEKDSGLLDSVLDTLLAPSGPGQSQPSPPACEVTSSWCLFGPELPEDP
     

Matching transcripts

 PGR-001 - ENSP00000325120 [100%]
PGR-002 - ENSP00000436561 [100%]
PGR-202 - ENSP00000482698 [100%]
  PGR-001 - ENSP00000325120 [100%]
PGR-006 - ENSP00000263463 [100%]
PGR-201 - ENSP00000481227 [100%]
PGR-202 - ENSP00000482698 [100%]
  PGR-001 - ENSP00000325120 [100%]
PGR-006 - ENSP00000263463 [100%]
PGR-201 - ENSP00000481227 [100%]
PGR-202 - ENSP00000482698 [100%]
     

Other gene match

          
ANTIGEN VIEW
PGR-001
PGR-002
PGR-006
PGR-201
PGR-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA004751

  

Antibody HPA008428

  

Antibody HPA017176

  

Antibody CAB000068

  

Antibody CAB055100

  

1.

 Prognostic and predictive significance of MYC and KRAS alterations in breast cancer from women treated with neoadjuvant chemotherapy
Pereira CB et al
PLoS One 2013;8(3):e60576
   Progesterone receptor negativity is an independent risk factor for relapse in patients with early stage endometrioid endometrial adenocarcinoma
Huvila J et al
Gynecol Oncol 2013;130(3):463-9
        
 

Contact

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The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.