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ME2

ANTIBODY INFORMATION
 
 

Antibody HPA008247

  

Antibody HPA008880

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA008247   HPA008880  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

   5  

VALIDATION SUMMARY
 
 

Antibody HPA008247

  

Antibody HPA008880

  
Immunocytochemistry

Supported   Uncertain  
Immunohistochemistry


Validated

Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008247

  

Antibody HPA008880

  

Standard validation

 Supported   Uncertain  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria & vesicles.  

Antibody dilution

 1:5   1:38  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008247

  

Antibody HPA008880

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA008880 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA008247 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in granular pattern in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008247

  

Antibody HPA008880

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 65.4   65.4, 53.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA008247

  

Antibody HPA008880

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA008247

  

Antibody HPA008880

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 126   129  

Antigen sequence

 
DGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDE
ELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKE
RTWRSEYDSLLPDVYEWPESASSPPV
  
KVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFD
KYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGA
GRLFTPDVIRAMASINERPVIFALSNPTA
 

Matching transcripts

 ME2-001 - ENSP00000321070 [100%]
  ME2-001 - ENSP00000321070 [100%]
ME2-002 - ENSP00000372384 [100%]
 

Other gene match

    
ANTIGEN VIEW
ME2-001
ME2-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA008247

  

Antibody HPA008880

  

1.

   Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells
MacDonald MJ et al
Arch Biochem Biophys 2009;488(2):100-4
  

2.

   Succinate is the controller of O2-/H2O2 release at mitochondrial complex I : negative modulation by malate, positive by cyanide
Zoccarato F et al
J Bioenerg Biomembr 2009;41(4):387-93
  

3.

   Chronic reduction of the cytosolic or mitochondrial NAD(P)-malic enzyme does not affect insulin secretion in a rat insulinoma cell line
Brown LJ et al
J Biol Chem 2009;284(51):35359-67
  

4.

   Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells
MacDonald MJ et al
Arch Biochem Biophys 2009;488(2):100-4
  

5.

   Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells
MacDonald MJ et al
Arch Biochem Biophys 2009;488(2):100-4
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.