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PIBF1

ANTIBODY INFORMATION
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA052269   HPA066120   HPA066125  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   14   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry

  Approved    
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to microtubule organizing center.     Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & microtubule organizing center.  

Antibody dilution

 1:119     1:32  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  

Standard validation

   Approved    

     

Figure description

   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.    

Expression

   RNA: detected in 37 tissues
Protein: detected in 78 cell types		    

Retrieval

   HIER pH6    

Antibody dilution

   1:450    

Literature conformity

   Partly consistent with extensive gene/protein characterization data.    

RNA consistency

   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 89.8, 83.1   89.8, 83.1, 25.1   89.8, 25.1  

Antibody dilution

 1:100   1:170   1:110  

PROTEIN ARRAY
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4750   1:2000   1:1150  

ANTIGEN INFORMATION
 
 

Antibody HPA052269

  

Antibody HPA066120

  

Antibody HPA066125

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   99   81  

Antigen sequence

 
QELMKQEMETILLRQKQLEETNLQLREKAGDVRRNLRDFELTEEQYIKLK
AFPEDQLSIPEYVSVRFYELVNPLRKEICELQVKKNILAEELSTNK
  
KELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRV
LQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESV
  
SLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQI
LVKMHSKHSENSLLLTKTEPKHVTENQKSKT
 

Matching transcripts

 PIBF1-001 - ENSP00000317144 [100%]
PIBF1-007 - ENSP00000478697 [100%]
  PIBF1-001 - ENSP00000317144 [100%]
PIBF1-007 - ENSP00000478697 [100%]
PIBF1-201 - ENSP00000483286 [93%]
  PIBF1-001 - ENSP00000317144 [100%]
PIBF1-201 - ENSP00000483286 [100%]
 

Other gene match

      
ANTIGEN VIEW
PIBF1-001
PIBF1-007
PIBF1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.