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SLC27A5

ANTIBODY INFORMATION
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007292   AMAb90572   AMAb90575  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 4   10   13  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Approved      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 6 cell types		   RNA: detected in 37 tissues
Protein: detected in 1 cell types		   RNA: detected in 37 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:1000   1:1200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 75.4, 66.8   75.4, 66.8, 10.9   75.4, 66.8, 10.9  

Antibody dilution

 1:250   1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  

Antigen

 Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 123      

Antigen sequence

 
RVLVVDPDLRESLEEILPKLQAENIRCFYLSHTSPTPGVGALGAALDAAP
SHPVPADLRAGITWRSPALFIYTSGTTGLPKPAILTHERVLQMSKMLSLS
GATADDVVYTVLPLYHVMGLVVG
     

Matching transcripts

 SLC27A5-001 - ENSP00000263093 [100%]
SLC27A5-002 - ENSP00000470368 [94%]
     

Other gene match

      
ANTIGEN VIEW
SLC27A5-001
SLC27A5-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA007292

  

Antibody CAB056160

  

Antibody CAB068239

  

1.

 Diagnosis in bile acid-CoA: amino acid N-acyltransferase deficiency
Hadžić N et al
World J Gastroenterol 2012;18(25):3322-6
      

2.

 Genetic defects in bile acid conjugation cause fat-soluble vitamin deficiency
Setchell KD et al
Gastroenterology 2013;144(5):945-955.e6; quiz e14-5
Application: IHC 		     
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.