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Antibody staining overlaps with antibody HPA070520.
Validated
Antibody staining overlaps with antibody HPA070213.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
Antibody dilution
1:167
1:53
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA070213
Antibody HPA070520
Antibody CAB019402
Standard validation
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA070213
Antibody HPA070520
Antibody CAB019402
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
156.8, 156.6, 152.4, 135.6
156.8, 156.6, 152.4, 135.6
156.8, 156.6, 152.4, 135.6
Antibody dilution
1:370
1:180
1:500
PROTEIN ARRAY
Antibody HPA070213
Antibody HPA070520
Antibody CAB019402
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
