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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Expression
RNA: detected in 36 tissues Protein: detected in 5 cell types
RNA: detected in 36 tissues Protein: detected in 5 cell types
RNA: detected in 36 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:150
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000685
Antibody HPA001295
Antibody CAB016760
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400731)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.2, 35.1
35.1
39.2, 35.1
Antibody dilution
1:500
1:250
1:500
PROTEIN ARRAY
Antibody HPA000685
Antibody HPA001295
Antibody CAB016760
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
