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FCN1

ANTIBODY INFORMATION
 
 

Antibody HPA000685

  

Antibody HPA001295

  

Antibody CAB016760

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   BioPorto Diagnostics A/S
  

Product name

 HPA000685   HPA001295   ABS 036-01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 1   1   4  

References

 2   1    

VALIDATION SUMMARY
 
 

Antibody HPA000685

  

Antibody HPA001295

  

Antibody CAB016760

  
Immunohistochemistry

Supported  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000685

  

Antibody HPA001295

  

Antibody CAB016760

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 40 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 5 cell types		   RNA: detected in 36 tissues
Protein: detected in 5 cell types		   RNA: detected in 36 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:150   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000685

  

Antibody HPA001295

  

Antibody CAB016760

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400731)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.2, 35.1   35.1   39.2, 35.1  

Antibody dilution

 1:500   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000685

  

Antibody HPA001295

  

Antibody CAB016760

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA000685

  

Antibody HPA001295

  

Antibody CAB016760

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 109   109    

Antigen sequence

 
LAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPG
PKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSC
ATGPRNCKD
  
QGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSL
TGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHE
SYANGINWS
  

											 
																				

																		

										
											
Matching transcripts

												
																					FCN1-001 - ENSP00000360871 [100%] 
FCN1-201 - ENSP00000479379 [100%] 

											 
																						FCN1-001 - ENSP00000360871 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FCN1-001
FCN1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA000685

												
										 
																				
											
											
											
Antibody HPA001295

												
										 
																				
											
											
											
Antibody CAB016760

												
										 
																		

																	

										
											
1.

												
																						From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

	Ek S et al

	Mol Cell Proteomics 2006;5(6):1072-81

			
											 
																							Fibrinogen-related proteins in ixodid ticks

	Sterba J et al

	Parasit Vectors 2011;4:127

			
											 
																						
											 
																				

																		

										
											
2.

												
																						LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood

	Okuzaki D et al

	BMC Clin Pathol 2011;11:9

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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