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Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nuclear membrane & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:200
1:38
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035000
Antibody HPA056750
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
Expression
RNA: detected in 36 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035000
Antibody HPA056750
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418147)
Target mass (kDa)
51.3
51.3, 5.7
Antibody dilution
1:250
1:130
PROTEIN ARRAY
Antibody HPA035000
Antibody HPA056750
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
