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TXLNG

ANTIBODY INFORMATION
 
 

Antibody HPA000297

  

Antibody HPA000841

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000297   HPA000841  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 1   1  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA000297

  

Antibody HPA000841

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000297

  

Antibody HPA000841

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

   1:80  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000297

  

Antibody HPA000841

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of bone marrow poietic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 42 cell types		   RNA: detected in 37 tissues
Protein: detected in 35 cell types		  

Retrieval

 HIER pH9   HIER pH6  

Antibody dilution

 1:1500   1:1000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000297

  

Antibody HPA000841

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 60.6, 46.3   60.6  

Antibody dilution

 1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000297

  

Antibody HPA000841

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA000297

  

Antibody HPA000841

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 115   121  

Antigen sequence

 
KALLQMAEEKTVRDKEYKALQIKLERLEKLCRALQTERNELNEKVEVLKE
QVSIKAAIKAANRDLATPVMQPCTALDSHKELNTSSKRALGAHLEAEPKS
QRSAVQKPPSTGSAP
  
VSPAYCTQESREEIPGGEARTDPPDGQQDSECNRNKEKTLGKEVLLLMQA
LNTLSTPEEKLAALCKKYADLLEESRSVQKQMKILQKKQAQIVKEKVHLQ
SEHSKAILARSKLESLCRELQ
 

Matching transcripts

 TXLNG-001 - ENSP00000369465 [100%]
TXLNG-201 - ENSP00000381222 [100%]
  TXLNG-001 - ENSP00000369465 [100%]
 

Other gene match

    
ANTIGEN VIEW
TXLNG-001
TXLNG-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA000297

  

Antibody HPA000841

  

1.

   Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
Mulder J et al
Mol Cell Proteomics 2009;8(7):1612-22
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.