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Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.
Immunohistochemical staining of human hippocampus shows strong positivity in astrocyte-like cells.
Expression
RNA: detected in 13 tissues Protein: detected in 41 cell types
RNA: detected in 13 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:1500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004011
Antibody CAB017779
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401803)
Target mass (kDa)
11.7, 8.4, 6.9
11.7, 9.8, 8.4, 6.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA004011
Antibody CAB017779
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.