LPCAT2 - Antibodies - The Human Protein Atlas

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LPCAT2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA007891

Antibody HPA008433

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA007891

HPA008433

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA007891

Antibody HPA008433

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA007891

Antibody HPA008433

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.

Antibody dilution

1:222

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA007891

Antibody HPA008433

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 44 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Standard validation

Supported

Figure description

Strong labelling of ependymal cells and modrate labelling of microglia.

WESTERN BLOT

Antibody HPA007891

Antibody HPA008433

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

60.2, 28.3

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA007891

Antibody HPA008433

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA007891

Antibody HPA008433

Antigen

Recombinant protein fragment

Length (aa)

133

Antigen sequence

FALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYIT
EEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKI
FTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPE

FALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYIT
EEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKI
FTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPE

Matching transcripts

LPCAT2-001 - ENSP00000262134 [100%]
LPCAT2-201 - ENSP00000483225 [100%]

Other gene match

ANTIGEN VIEW

LPCAT2-001	LPCAT2-201

RELEVANT PUBLICATIONS

Antibody HPA007891

Antibody HPA008433

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.