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L2HGDH

ANTIBODY INFORMATION
 
 

Antibody HPA065409

  

Antibody HPA069708

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA065409   HPA069708  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA065409

  

Antibody HPA069708

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA065409

  

Antibody HPA069708

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA069708.		  
Validated

Antibody staining overlaps with antibody HPA065409.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.   Immunofluorescent staining of human cell line RT4 shows localization to mitochondria.  

Antibody dilution

 1:200   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA065409

  

Antibody HPA069708

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human duodenum shows strong positivity with a granular a pattern in glandular cells.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 54 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1500    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA065409

  

Antibody HPA069708

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 50.3, 48.5   50.3  

Antibody dilution

 1:700   1:840  

PROTEIN ARRAY
 
 

Antibody HPA065409

  

Antibody HPA069708

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:8400   1:10000  

ANTIGEN INFORMATION
 
 

Antibody HPA065409

  

Antibody HPA069708

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 77   70  

Antigen sequence

 
KKEPYCRGLMAIDCPHTGIVDYRQVALSFAQDFQEAGGSVLTNFEVKGIE
MAKESPSRSIDGMQYPIVIKNTKGEEI
  
ISDILRGPAGVRAQALDRDGNLVEDFVFDAGVGDIGNRILHVRNAPSPAA
TSSIAISGMIADEVQQRFEL
 

Matching transcripts

 L2HGDH-001 - ENSP00000267436 [100%]
L2HGDH-002 - ENSP00000261699 [100%]
L2HGDH-003 - ENSP00000405559 [100%]
  L2HGDH-001 - ENSP00000267436 [100%]
L2HGDH-003 - ENSP00000405559 [100%]
 

Other gene match

    
ANTIGEN VIEW
L2HGDH-001
L2HGDH-002
L2HGDH-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.