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DNM1L

ANTIBODY INFORMATION
 
 

Antibody HPA039324

  

Antibody CAB009952

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA039324   sc-32898  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA039324

  

Antibody CAB009952

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039324

  

Antibody CAB009952

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:33   1:16  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039324

  

Antibody CAB009952

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human lateral ventricle shows strong nuclear positivity in glial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:125   1:65  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039324

  

Antibody CAB009952

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 83.4, 82.1, 81.9, 80.5, 79.6, 79.4, 78.1, 60   83.4, 82.1, 81.9, 80.5, 79.6, 79.4, 78.1, 60, 29, 19.1, 18.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA039324

  

Antibody CAB009952

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA039324

  

Antibody CAB009952

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 81    

Antigen sequence

 
EADGKVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMP
ASPQKGHAVNLLDVPVPVARKLSAREQRDCE
  

											 
																				

																		

										
											
Matching transcripts

												
																					DNM1L-008 - ENSP00000448610 [100%] 
DNM1L-010 - ENSP00000370388 [100%] 
DNM1L-004 - ENSP00000350948 [95%] 
DNM1L-005 - ENSP00000266481 [95%] 
DNM1L-001 - ENSP00000415131 [94%] 
DNM1L-002 - ENSP00000449089 [88%] 
DNM1L-003 - ENSP00000450399 [88%] 
DNM1L-201 - ENSP00000404160 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DNM1L-001
DNM1L-002
DNM1L-003
DNM1L-004
DNM1L-005
DNM1L-008
DNM1L-010
DNM1L-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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