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AURKA

ANTIBODY INFORMATION
 
 

Antibody HPA002636

  

Antibody CAB001454

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA002636   NCL-L-AK2  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 4   1  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA002636

  

Antibody CAB001454

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002636

  

Antibody CAB001454

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.    

Antibody dilution

 1:71    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002636

  

Antibody CAB001454

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows moderate positivity in a subset of cells in seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells of seminiferus ducts.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 23 cell types		   RNA: detected in 34 tissues
Protein: detected in 24 cell types		  

Retrieval

 HIER pH6   HIER pH9  

Antibody dilution

 1:100   1:25  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002636

  

Antibody CAB001454

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.8, 39.5, 30.7, 21.2, 16.2   45.8, 39.5, 30.7, 21.2, 16.2, 11.5, 8.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002636

  

Antibody CAB001454

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA002636

  

Antibody CAB001454

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 141    

Antigen sequence

 
ISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVP
LQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPEN
NPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYL
   

Matching transcripts

 AURKA-001 - ENSP00000216911 [99%]
AURKA-002 - ENSP00000393452 [99%]
AURKA-003 - ENSP00000379251 [99%]
AURKA-004 - ENSP00000321591 [99%]
AURKA-005 - ENSP00000360407 [99%]
AURKA-006 - ENSP00000379249 [99%]
AURKA-009 - ENSP00000388073 [99%]
AURKA-010 - ENSP00000379247 [99%]
AURKA-011 - ENSP00000379243 [99%]
AURKA-201 - ENSP00000379250 [99%]
AURKA-008 - ENSP00000405042 [98%]
   

Other gene match

    
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Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.