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ASAP3

ANTIBODY INFORMATION
 
 

Antibody HPA020537

  

Antibody HPA020546

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020537   HPA020546  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA020537

  

Antibody HPA020546

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020537

  

Antibody HPA020546

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.  

Antibody dilution

   1:149  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020537

  

Antibody HPA020546

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non-germinal center cells.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 10 cell types		   RNA: detected in 36 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:500  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020537

  

Antibody HPA020546

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 99.2, 97.8, 43.7   99.2, 97.8, 43.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020537

  

Antibody HPA020546

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020537

  

Antibody HPA020546

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 62   107  

Antigen sequence

 
EAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAIC
NRDLLSVLEAFA
  
KKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLL
KLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRT
LVQGCAR
 

Matching transcripts

 ASAP3-001 - ENSP00000338769 [100%]
ASAP3-005 - ENSP00000436150 [100%]
ASAP3-011 - ENSP00000408826 [100%]
  ASAP3-001 - ENSP00000338769 [100%]
ASAP3-005 - ENSP00000436150 [100%]
ASAP3-011 - ENSP00000408826 [100%]
 

Other gene match

    
ANTIGEN VIEW
ASAP3-001
ASAP3-005
ASAP3-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.