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ANKRD10

ANTIBODY INFORMATION
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA038878   HPA038879   sc-83976  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 13   12   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.    

Antibody dilution

   1:110    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.   Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human salivary gland shows strong granular/cytoplasmic positivity in ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 29 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:450   1:70  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 44.8   44.8   44.8, 23.5, 13.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:12000    

ANTIGEN INFORMATION
 
 

Antibody HPA038878

  

Antibody HPA038879

  

Antibody CAB034211

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 98   99    

Antigen sequence

 
SSQGSLCISGTEEPEKTLRANPELCGSLHLNGSPSSCIASRPSWVEDIGD
NLYYGHYHGFGDTAESIPELNSVVEHSKSVKVQERYDSAVLGTMHLHH
  
DCEGETPIHKAARSGSLECISALVANGAHVDLRNASGLTAADIAQTQGFQ
ECAQFLLNLQNCHLNHFYNNGILNGGHQNVFPNHISVGTNRKRCLEDSE
  

											 
																				

																		

										
											
Matching transcripts

												
																					ANKRD10-003 - ENSP00000267339 [100%] 

											 
																						ANKRD10-003 - ENSP00000267339 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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