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Antibody staining overlaps with antibody HPA037544.
Validated
Antibody staining overlaps with antibody HPA037543.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:130
1:54
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037543
Antibody HPA037544
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and neuropil was distinctly stained. .
Expression
RNA: detected in 12 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037543
Antibody HPA037544
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
233.1
233.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037543
Antibody HPA037544
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
