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ARHGAP28

ANTIBODY INFORMATION
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030413   HPA030414   HPA030415  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   7   7  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cell junctions.   Immunofluorescent staining of human cell line CACO-2 shows localization to cell junctions.    

Antibody dilution

 1:114   1:81    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in subsets of cells in seminiferus ducts.   Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in a subset of cells in seminiferus ducts.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 13 cell types		   RNA: detected in 34 tissues
Protein: detected in 11 cell types		   RNA: detected in 34 tissues
Protein: detected in 20 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:100   1:50  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 82.1, 75.8, 64.6, 64.2, 62   82.1, 75.8, 64.6, 64.2, 62, 12   82.1, 75.8, 13, 8.1  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   85   67  

Antigen sequence

 
VLPVHSNGSPEPGQPVQNAISDDDFLEKNIPPEAEELSFEVSYSEMVTEA
LKRNKLKKSEIKKEDYVLTKFNVQKTRFGL
  
KQSIRDVRDIFGVSESPPRDTCGNHTNQLDGTKEERELPRVIKTSGSMPD
DASLNSTTLSDASQDKEGSFAVPRSDSVAILETIP
  
KSIPRCRRINRMLSNESLHPPAFSRSNSEASVDSASMEDFWREIESIKDS
SMGGQEEPPPAEVTPVD
 

Matching transcripts

 ARHGAP28-001 - ENSP00000392660 [100%]
ARHGAP28-002 - ENSP00000435990 [100%]
ARHGAP28-003 - ENSP00000262227 [100%]
ARHGAP28-004 - ENSP00000437262 [100%]
ARHGAP28-006 - ENSP00000372964 [100%]
ARHGAP28-201 - ENSP00000313506 [100%]
  ARHGAP28-001 - ENSP00000392660 [100%]
ARHGAP28-003 - ENSP00000262227 [100%]
ARHGAP28-006 - ENSP00000372964 [100%]
ARHGAP28-015 - ENSP00000462912 [100%]
ARHGAP28-201 - ENSP00000313506 [100%]
ARHGAP28-004 - ENSP00000437262 [93%]
ARHGAP28-002 - ENSP00000435990 [80%]
  ARHGAP28-006 - ENSP00000372964 [100%]
ARHGAP28-007 - ENSP00000464141 [100%]
ARHGAP28-003 - ENSP00000262227 [84%]
ARHGAP28-005 - ENSP00000433390 [84%]
 

Other gene match

      
ANTIGEN VIEW
ARHGAP28-001
ARHGAP28-002
ARHGAP28-003
ARHGAP28-004
ARHGAP28-005
ARHGAP28-006
ARHGAP28-007
ARHGAP28-015
ARHGAP28-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA030413

  

Antibody HPA030414

  

Antibody HPA030415

  

1.

 Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP)
Fagerberg L et al
J Proteome Res 2013;12(6):2439-48
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.