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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, nuclear membrane & vesicles.
Antibody dilution
1:141
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA047117
Antibody HPA060198
Standard validation
Approved
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells while endothelial cells and ganglion cells were moderately stained.
Expression
RNA: detected in 36 tissues Protein: detected in 81 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047117
Antibody HPA060198
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
65, 61.8, 59.3, 56.1, 20.6
65, 61.8, 59.3, 56.1, 49.8
Antibody dilution
1:130
1:470
PROTEIN ARRAY
Antibody HPA047117
Antibody HPA060198
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
