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NSFL1C

ANTIBODY INFORMATION
 
 

Antibody HPA047108

  

Antibody HPA050628

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047108   HPA050628  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   10  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047108

  

Antibody HPA050628

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047108

  

Antibody HPA050628

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA050628.		  
Validated

Antibody staining overlaps with antibody HPA047108.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:200   1:189  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047108

  

Antibody HPA050628

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA050628 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA047108 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:60000   1:1100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047108

  

Antibody HPA050628

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 40.8, 40.6, 37.3   40.8, 40.6, 37.3  

Antibody dilution

 1:3550   1:940  

PROTEIN ARRAY
 
 

Antibody HPA047108

  

Antibody HPA050628

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:29600   1:7000  

ANTIGEN INFORMATION
 
 

Antibody HPA047108

  

Antibody HPA050628

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   72  

Antigen sequence

 
MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDI
VTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDADEE
  
EEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVER
VTKSPGETSKPRPFAGGGYRLG
 

Matching transcripts

 NSFL1C-002 - ENSP00000216879 [99%]
NSFL1C-004 - ENSP00000338643 [99%]
NSFL1C-010 - ENSP00000418529 [99%]
  NSFL1C-002 - ENSP00000216879 [100%]
NSFL1C-010 - ENSP00000418529 [97%]
NSFL1C-004 - ENSP00000338643 [86%]
 

Other gene match

    
ANTIGEN VIEW
NSFL1C-002
NSFL1C-004
NSFL1C-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.