SNX5 - Antibodies - The Human Protein Atlas

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SNX5

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA051187

Antibody CAB020679

Provider

Atlas Antibodies
Sigma-Aldrich

SDIX

Product name

HPA051187

2991.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA051187

Antibody CAB020679

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA051187

Antibody CAB020679

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & cytosol.

Antibody dilution

1:19

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA051187

Antibody CAB020679

Standard validation

Approved

Figure description

Immunohistochemical staining of human thyroid gland shows moderate cytoplasmiuc positivity in glandular cells.

Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 79 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:75

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA051187

Antibody CAB020679

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

46.8, 31, 19.6

46.8, 31, 26.7, 19.6, 13.3, 5.9, 3.5

Antibody dilution

1:360

1:500

PROTEIN ARRAY

Antibody HPA051187

Antibody CAB020679

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2850

ANTIGEN INFORMATION

Antibody HPA051187

Antibody CAB020679

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

FFEQEKNFLINYYNRIKDSCVKADKMTRSHKNVADDYIHTAACLHSLALE
EPTVIKKYLLKVAELFEKLRKVEGRV

Matching transcripts

SNX5-001 - ENSP00000366998 [100%]
SNX5-013 - ENSP00000404448 [100%]
SNX5-014 - ENSP00000479021 [100%]
SNX5-019 - ENSP00000366988 [100%]

Other gene match

ANTIGEN VIEW

SNX5-001	SNX5-013	SNX5-014	SNX5-019

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.