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KIF16B

ANTIBODY INFORMATION
 
 

Antibody HPA051198

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051198  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA051198

  
Immunocytochemistry

Uncertain  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051198

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to mitochondria.  

Antibody dilution

 1:132  

Literature conformity

 The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051198

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:600  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051198

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 159.8, 152  

Antibody dilution

 1:660  

PROTEIN ARRAY
 
 

Antibody HPA051198

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5300  

ANTIGEN INFORMATION
 
 

Antibody HPA051198

  

Antigen

 Recombinant protein fragment  

Length (aa)

 91  

Antigen sequence

 
KLVNLEKDLVQQKDILKKEVQEEQEILECLKCEHDKESRLLEKHDESVTD
VTEVPQDFEKIKPVEYRLQYKERQLQYLLQNHLPTLLEEKQ
 

Matching transcripts

 KIF16B-001 - ENSP00000384164 [100%]
KIF16B-003 - ENSP00000347076 [100%]
 

Other gene match

  
ANTIGEN VIEW
KIF16B-001
KIF16B-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.