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Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane.
Antibody dilution
1:56
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA067188
Antibody CAB031489
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human gallbladder shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 80 cell types
Retrieval
HIER pH6
Antibody dilution
1:600
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA067188
Antibody CAB031489
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
38
38
Antibody dilution
1:190
1:500
PROTEIN ARRAY
Antibody HPA067188
Antibody CAB031489
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
