ITGA6 - Antibodies - The Human Protein Atlas

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ITGA6

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA012696

HPA027582

sc-10730

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA027582 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA012696 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human rectum shows moderate cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in cortical cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 41 cell types

RNA: detected in 37 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:425

1:150

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

126.6, 122.1, 121.7, 119.5, 106.6

126.6, 122.1, 121.7, 119.5, 106.6, 29.7, 25.5

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

137

Antigen sequence

APYDDLGKVFIYHGSANGINTKPTQVLKGISPYFGYSIAGNMDLDRNSYP
DVAVGSLSDSVTIFRSRPVINIQKTITVTPNRIDLRQKTACGAPSGICLQ
VKSCFEYTANPAGYNPSISIVGTLEAEKERRKSGLSS

RVINLGKPLTNLGTATLNIQWPKEISNGKWLLYLVKVESKGLEKVTCEPQ
KEINSLNLTESHNSRKKREITEKQIDDNRKFSLFAERKYQT

Matching transcripts

ITGA6-001 - ENSP00000264107 [100%]
ITGA6-003 - ENSP00000386614 [100%]
ITGA6-004 - ENSP00000386896 [100%]
ITGA6-010 - ENSP00000406694 [100%]
ITGA6-011 - ENSP00000394169 [100%]

Other gene match

ANTIGEN VIEW

ITGA6-001	ITGA6-003	ITGA6-004	ITGA6-010	ITGA6-011

RELEVANT PUBLICATIONS

Antibody HPA012696

Antibody HPA027582

Antibody CAB009009

Identification of integrins alpha6 and beta7 as c-Jun- and transformation-relevant genes in highly invasive fibrosarcoma cells
Kielosto M et al
Int J Cancer 2009;125(5):1065-73

Multiplex flow cytometry barcoding and antibody arrays identify surface antigen profiles of primary and metastatic colon cancer cell lines
Sukhdeo K et al
PLoS One 2013;8(1):e53015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.