CPA1 - Antibodies - The Human Protein Atlas

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CPA1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA021836

Antibody HPA052215

Antibody CAB025197

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA021836

HPA052215

AF2856

Host species

Rabbit

Goat

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA021836

Antibody HPA052215

Antibody CAB025197

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA021836

Antibody HPA052215

Antibody CAB025197

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA052215 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA021836 across 45 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.

Expression

RNA: detected in 18 tissues
Protein: detected in 5 cell types

RNA: detected in 18 tissues
Protein: detected in 1 cell types

RNA: detected in 18 tissues
Protein: detected in 19 cell types

Retrieval

HIER pH6

Antibody dilution

1:1500

1:600

1:120

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA021836

Antibody HPA052215

Antibody CAB025197

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

47.1, 37.4, 30.2, 11.9

47.1

47.1, 37.4, 30.2, 20.9, 11.9

Antibody dilution

1:250

1:580

1:500

PROTEIN ARRAY

Antibody HPA021836

Antibody HPA052215

Antibody CAB025197

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:4650

ANTIGEN INFORMATION

Antibody HPA021836

Antibody HPA052215

Antibody CAB025197

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

MIEDVQSLLDEEQEQMFAFRSRARSTDTFNYATYHTLEEIYDFLDLLVAE
NPHLVSKIQIGNTYEGRPIYVLKF

DFVGHQVLRISVADEAQVQKVKELEDLEHLQLDFWRGPAHPGSPIDVRVP
FPSIQAVKIFLESHGISYET

Matching transcripts

CPA1-001 - ENSP00000011292 [100%]
CPA1-003 - ENSP00000419497 [100%]
CPA1-005 - ENSP00000420218 [100%]
CPA1-007 - ENSP00000419408 [100%]

CPA1-001 - ENSP00000011292 [100%]

Other gene match

ANTIGEN VIEW

CPA1-001	CPA1-003	CPA1-005	CPA1-007

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.