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HNRNPC

ANTIBODY INFORMATION
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051075   sc-15386   AMAb91010   AMAb91012  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 12   2   14   14  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  
Immunocytochemistry


Validated
   Supported      
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot


Validated
   Supported   Uncertain   Uncertain  
Protein array

Approved        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		        

 

Relative fluorescence intensity (RFI)

        

Figure description

 Nuclear region of segmented cells in 10x-images        

Antibody dilution

 1:28        
 

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.      

Antibody dilution

 1:28   1:112      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.   Immunohistochemical staining of human tonsil shows strong positivity in germinal center cells and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:450   1:1500   1:500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		        

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		        

Target mass (kDa)

 33.7, 33.6, 25.3, 20.4, 19.2        

Loading control

 Total protein image        

Antibody dilution

 1:63        
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 33.7, 33.6, 25.3, 20.4, 19.2   33.7, 33.6, 32.3, 32.2, 32, 31.9, 28.9, 27.8, 25.3, 25.2, 23.8, 20.4, 20.3, 19.2, 14, 13.3, 12.8, 7.3, 5.9   33.7, 33.6, 32.3, 32.2, 32, 31.9, 28.9, 27.8, 25.3, 25.2, 23.8, 20.4, 20.3, 19.2, 14, 13.3, 12.8, 7.3, 5.9   33.7, 33.6, 32.3, 32.2, 32, 31.9, 28.9, 27.8, 25.3, 25.2, 23.8, 20.4, 20.3, 19.2, 14, 13.3, 12.8, 7.3, 5.9  

Antibody dilution

 1:140   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		        

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:1150        

ANTIGEN INFORMATION
 
 

Antibody HPA051075

  

Antibody CAB005223

  

Antibody CAB075755

  

Antibody CAB075756

  

Antigen

 Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 20        

Antigen sequence

 
AEMYGSVTEHPSPSPLLSSS
       

Matching transcripts

 HNRNPC-005 - ENSP00000451291 [100%]
HNRNPC-006 - ENSP00000404848 [100%]
HNRNPC-007 - ENSP00000452276 [100%]
HNRNPC-010 - ENSP00000450790 [100%]
HNRNPC-018 - ENSP00000452214 [100%]
HNRNPC-020 - ENSP00000451187 [100%]
HNRNPC-028 - ENSP00000452213 [100%]
HNRNPC-033 - ENSP00000452185 [100%]
       

Other gene match

        
ANTIGEN VIEW
HNRNPC-005
HNRNPC-006
HNRNPC-007
HNRNPC-010
HNRNPC-018
HNRNPC-020
HNRNPC-028
HNRNPC-033
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.