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Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells segments.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cell segments and in subsets of nerve fibers.
Expression
RNA: detected in 7 tissues Protein: detected in 12 cell types
RNA: detected in 7 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:225
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA042955
Antibody HPA060190
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
146.7, 142.8, 108.1, 87, 70.1
146.7, 142.8, 108.1, 87, 70.1
Antibody dilution
1:250
1:90
PROTEIN ARRAY
Antibody HPA042955
Antibody HPA060190
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
