Cell atlas - PHGDH - The Human Protein Atlas

GENERAL INFORMATION
Gene name	PHGDH
Gene description	Phosphoglycerate dehydrogenase
Protein class	Disease related genes Enzymes Plasma proteins Potential drug targets Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	2
	SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the plasma membrane, cytosol & nucleoplasm.
RNA cell category	Mixed
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated), Plasma membrane (validated), Cytosol (validated)
Single-cell variation	Single-cell variation in protein expression observed.
Mouse	Localized to the Cytosol in mouse cells.

DATA RELIABILITY
Reliability score	Validated based on 3 antibodies. CAB003681 , HPA021241 , HPA024031
	SHOW MORE

ASSAYS

RNA expression

Human cells

Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Mixed Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the plasma membrane, cytosol & nucleoplasm.

Main location

Nucleoplasm (validated), Plasma membrane (validated), Cytosol (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation
HPA021241	A-431	Nucleoplasm
		Plasma membrane
		Cytosol
HPA021241	U-2 OS	Plasma membrane
HPA021241	U-2 OS	Cytosol
HPA021241	U-251 MG	Plasma membrane
HPA021241	U-251 MG	Cytosol
HPA024031	MCF7	Nucleoplasm	Intensity
		Plasma membrane	Intensity
		Cytosol
HPA024031	PC-3	Nucleoplasm
		Plasma membrane
		Cytosol
HPA024031	U-2 OS	Nucleoplasm
HPA024031	U-2 OS	Cytosol
CAB003681	A-431	Cytosol	Intensity
CAB003681	U-2 OS	Cytosol
CAB003681	U-251 MG	Cytosol

MOUSE CELLS

Main location

Cytosol

Mouse gene

ENSMUSG00000053398 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA024031	NIH 3T3	Cytosol

GENE INFORMATION
Gene name	PHGDH (HGNC Symbol)
Synonyms	PDG, PGDH, SERA
Description	Phosphoglycerate dehydrogenase (HGNC Symbol)
Entrez gene summary	This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
Chromosome	1
Cytoband	p12
Chromosome location (bp)	119659798 - 119744215
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000092621 (version 83.38)
Entrez gene	26227
UniProt	O43175 (UniProt - Evidence at protein level)
neXtProt	NX_O43175
Antibodypedia	PHGDH antibodies

PROTEIN BROWSER

PHGDH-001	PHGDH-002

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
PHGDH-001 ENSP00000358417 ENST00000369409	O43175 [Direct mapping] D-3-phosphoglycerate dehydrogenase Show all	Enzymes ENZYME proteins Oxidoreductases SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Plasma proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004617 [phosphoglycerate dehydrogenase activity] GO:0005829 [cytosol] GO:0006541 [glutamine metabolic process] GO:0006544 [glycine metabolic process] GO:0006563 [L-serine metabolic process] GO:0006564 [L-serine biosynthetic process] GO:0006566 [threonine metabolic process] GO:0007420 [brain development] GO:0008152 [metabolic process] GO:0008652 [cellular amino acid biosynthetic process] GO:0009055 [electron carrier activity] GO:0009070 [serine family amino acid biosynthetic process] GO:0009448 [gamma-aminobutyric acid metabolic process] GO:0010468 [regulation of gene expression] GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor] GO:0019530 [taurine metabolic process] GO:0021510 [spinal cord development] GO:0021782 [glial cell development] GO:0021915 [neural tube development] GO:0022008 [neurogenesis] GO:0031175 [neuron projection development] GO:0034641 [cellular nitrogen compound metabolic process] GO:0043209 [myelin sheath] GO:0044281 [small molecule metabolic process] GO:0051287 [NAD binding] GO:0055114 [oxidation-reduction process] GO:0070062 [extracellular exosome] GO:0070314 [G1 to G0 transition] Show all	533 aa 56.6 kDa	No	0
PHGDH-002 ENSP00000358415 ENST00000369407	Q5SZU1 [Direct mapping] D-3-phosphoglycerate dehydrogenase Show all	SPOCTOPUS predicted membrane proteins SignalP predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004617 [phosphoglycerate dehydrogenase activity] GO:0006564 [L-serine biosynthetic process] GO:0008152 [metabolic process] GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor] GO:0051287 [NAD binding] GO:0055114 [oxidation-reduction process] Show all	499 aa 53.1 kDa	No	0