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Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
Antibody dilution
1:10
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027248
Antibody HPA027461
Standard validation
Approved
Figure description
Immunohistochemical staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.
Expression
RNA: detected in 9 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027248
Antibody HPA027461
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415231)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415231)
Target mass (kDa)
49.7
49.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA027248
Antibody HPA027461
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
