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Antibody staining overlaps with antibody HPA026312.
Validated
Antibody staining overlaps with antibody HPA026305.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
Antibody dilution
1:24
1:7
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA026305
Antibody HPA026312
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
151.1, 150.4, 145.3
151.1, 150.4, 145.3, 144.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA026305
Antibody HPA026312
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.