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NUP188

ANTIBODY INFORMATION
 
 

Antibody HPA061750

  

Antibody HPA063942

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA061750   HPA063942  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA061750

  

Antibody HPA063942

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA061750

  

Antibody HPA063942

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.   Immunofluorescent staining of human cell line SiHa shows localization to nucleus & nucleoli.  

Antibody dilution

 1:27   1:8  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA061750

  

Antibody HPA063942

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:15    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA061750

  

Antibody HPA063942

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 196   196  

Antibody dilution

 1:90   1:80  

PROTEIN ARRAY
 
 

Antibody HPA061750

  

Antibody HPA063942

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA061750

  

Antibody HPA063942

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 120   92  

Antigen sequence

 
YFSALILVEGMDIESLHKCALDDRRELHQFAQDGLICQDMDCLMLTFGDI
PHHAPVLLAWALLRHTLNPEETSSVVRKIGGTAIQLNVFQYLTRLLQSLA
SGGNDCTTSTACMCVYGLLS
  
APMSVYACLGNDAAAIRDAFLTRLQSKIEDMRIKVMILEFLTVAVETQPG
LIELFLNLEVKDGSDGSKEFSLGMWSCLHAVLELIDSQQQDR
 

Matching transcripts

 NUP188-001 - ENSP00000361658 [100%]
  NUP188-001 - ENSP00000361658 [100%]
 

Other gene match

    
ANTIGEN VIEW
NUP188-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.