SORBS1 - Antibodies - The Human Protein Atlas

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SORBS1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027559

Antibody HPA043084

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027559

HPA043084

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA027559

Antibody HPA043084

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027559

Antibody HPA043084

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & focal adhesion sites.

Antibody dilution

1:100

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027559

Antibody HPA043084

Standard validation

Approved

Figure description

Immunohistochemical staining of human heart muscle shows strong membranous and cytoplasmic positivity in myocytes.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 60 cell types

RNA: detected in 37 tissues
Protein: detected in 59 cell types

Retrieval

HIER pH6

Antibody dilution

1:450

1:50

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027559

Antibody HPA043084

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

143.7, 142.5, 139.4, 127.3, 111.8, 101.1, 91.1, 90.2, 87.2, 76.6, 20.6

143.7

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA027559

Antibody HPA043084

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:30000

1:12000

ANTIGEN INFORMATION

Antibody HPA027559

Antibody HPA043084

Antigen

Recombinant protein fragment

Length (aa)

137

Antigen sequence

DSSPLLNEVSSSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQ
QKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAKDDSRRVVKST
QDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNR

ESLLESTKLHPKEMDGMNSSGVYASPTCSNMAHHALSFRGLVPSEPLSTC
SDDVDRCSNISTDSREGSGGSVHGDFPKHRLNKCKG

Matching transcripts

SORBS1-001 - ENSP00000302556 [100%]
SORBS1-002 - ENSP00000360295 [100%]
SORBS1-004 - ENSP00000355136 [100%]
SORBS1-005 - ENSP00000277982 [100%]
SORBS1-006 - ENSP00000360291 [100%]
SORBS1-007 - ENSP00000360285 [100%]
SORBS1-008 - ENSP00000277984 [100%]
SORBS1-009 - ENSP00000360283 [100%]
SORBS1-015 - ENSP00000360271 [100%]
SORBS1-201 - ENSP00000360292 [100%]
SORBS1-202 - ENSP00000360293 [100%]
SORBS1-017 - ENSP00000475901 [93%]
SORBS1-016 - ENSP00000473813 [85%]

SORBS1-017 - ENSP00000475901 [100%]

Other gene match

ANTIGEN VIEW

SORBS1-001	SORBS1-002	SORBS1-004	SORBS1-005	SORBS1-006	SORBS1-007	SORBS1-008	SORBS1-009	SORBS1-015	SORBS1-016	SORBS1-017	SORBS1-201	SORBS1-202

RELEVANT PUBLICATIONS

Antibody HPA027559

Antibody HPA043084

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.