MYO3A - Antibodies - The Human Protein Atlas

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MYO3A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA048951

Antibody HPA051893

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA048951

HPA051893

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA048951

Antibody HPA051893

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA048951

Antibody HPA051893

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & cytoplasmic bodies.

Antibody dilution

1:42

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA048951

Antibody HPA051893

Standard validation

Approved

Figure description

Immunohistochemical staining of human retina shows moderate positivity in photoreceptor and ganglion cells.

Expression

RNA: detected in 1 tissues
Protein: detected in 22 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

No internal RNA expression data available for correlation.

WESTERN BLOT

Antibody HPA048951

Antibody HPA051893

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

186.2

Antibody dilution

1:240

1:210

PROTEIN ARRAY

Antibody HPA048951

Antibody HPA051893

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1950

1:1700

ANTIGEN INFORMATION

Antibody HPA048951

Antibody HPA051893

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

IIIQSAARGHLVRKQRKEIVDMKNTAVTTIQTSDQEFDYKKNFENTRESF
VKKQAENAISANERFISAPNNKGSVSVVKTSTFKPEEETTNAV

KRRPRKDSQGKLLDLEDFYYKEFLPSRSGPKEHSPSLRERRPQQELQNQC
IKANERCWAAESPEKEEEREPAANPYDFR

Matching transcripts

MYO3A-001 - ENSP00000265944 [100%]

Other gene match

ANTIGEN VIEW

MYO3A-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.