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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & actin filaments.
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane & actin filaments.
Antibody dilution
1:185
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA017959
Antibody HPA030549
Standard validation
Approved
Figure description
Immunohistochemical staining of human salivary gland shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017959
Antibody HPA030549
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413585)
Target mass (kDa)
62.8, 62.7, 53.3
62.8, 62.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA017959
Antibody HPA030549
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
