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KCNK6

ANTIBODY INFORMATION
 
 

Antibody HPA040184

  

Antibody CAB022637

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA040184   AB5572  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   5  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA040184

  

Antibody CAB022637

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040184

  

Antibody CAB022637

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.  

Antibody dilution

   1:25  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040184

  

Antibody CAB022637

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 74 cell types		   RNA: detected in 36 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040184

  

Antibody CAB022637

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 33.7   33.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA040184

  

Antibody CAB022637

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA040184

  

Antibody CAB022637

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 56    

Antigen sequence

 
LQTFRHVSDLHGLTELILLPPPCPASFNADEDDRVDILGPQPESHQQLSA
SSHTDY
  

											 
																				

																		

										
											
Matching transcripts

												
																					KCNK6-001 - ENSP00000263372 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KCNK6-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA040184

												
										 
																				
											
											
											
Antibody CAB022637

												
										 
																		

																	

										
											
1.

												
																						Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium

	Kim SH et al

	Sci Rep 2015;5:18110

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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